TABLE 1.
Sedimentation velocity AUC parameters.
| IDP | Mseq (kDa) | s 20,w (dc/dt) | Mexp (kDa) (dc/dt) | s 20,w (c(s)) | f/f 0 (c(s)) | Mexp (kDa) (c(s)) | R s (Å) | R h (Å) (SEC) | R h (PPII charge) |
|---|---|---|---|---|---|---|---|---|---|
| Aap‐PGR | 13.1 | 1.02 | 14.3 | 1.06 | 2.12 | 14.4 | 33.9 | 37.1 | 37.84 |
| SasG‐PGR | 7.5 | 0.76 | 8.9 | 0.80 | 2.03 | 9.3 | 28.1 | 24.8 | 24.43 |
| Aap‐Arpts | 19.1 | 1.25 | 25.6 | 1.30 | 2.52 | 22.2 | 46.2 | 40.8 | 44.06 |
| SdrC‐SD | 6.4 | 0.91 | 8.4 | 0.97 | 1.93 | 8.1 | 24.7 | 21.1 | 22.15 |
Note: Data were analyzed using the −dc/dt fitting in DCDT+ and by SEDFIT's continuous c(s) distribution model (Brown & Schuck, 2006) and are presented in Figure 3. The Stokes‐Einstein radius, R s , is from SEDFIT, R h was determined experimentally by SEC, and the predicted hydrodynamic radius, R h (PPII charge), is from Table S2, shown here for comparison.