TABLE 3.
Stalk regions in adhesin‐like staphylococcal CWA proteins.
| Protein | Ligand(s) a | Family b | Length: LCR (Total) | SD repeats | Prolines | Das‐Pappu region | FCR c | FPR | f PPII d | NCPR | Kappa | SCD e | Omega |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SD‐rich | |||||||||||||
| SdrC | Self, β‐neurexin | Clf‐Sdr | 170 (193) | 83 | 2 | 4 | 0.505 | 0.010 | 0.289 | −0.479 | 0.056 | 110.90 | 0.00195 |
| SdrD | Desmoglein‐1 | Clf‐Sdr | 142 (165) | 71 | 2 | 4 | 0.466 | 0.012 | 0.290 | −0.415 | 0.107 | 82.04 | 0.00348 |
| SdrE | Factor H | Clf‐Sdr | 158 (181) | 79 | 2 | 4 | 0.467 | 0.011 | 0.288 | −0.406 | 0.125 | 99.61 | 0.00299 |
| SdrF (S. epi) | Collagen | Clf‐Sdr | 170 (193) | 84 | 2 | 4 | 0.470 | 0.010 | 0.289 | −0.414 | 0.114 | 113.55 | 0.00280 |
| SdrG (S. epi) | Fibrinogen (β) | Clf‐Sdr | 140 (163) | 69 | 2 | 4 | 0.472 | 0.012 | 0.293 | −0.420 | 0.106 | 79.96 | 0.00264 |
| Pls | Unknown | G5‐E | 294 (309) | 112 | 1 | 4 | 0.466 | 0.003 | 0.286 | −0.405 | 0.125 | 325.06 | 0.00298 |
| ClfA | Fibrinogen (γ), Factor I | Clf‐Sdr | 323 (352) | 130 | 10 | 4 | 0.423 | 0.028 | 0.289 | −0.401 | 0.101 | 260.80 | 0.03617 |
| ClfB | Fibrinogen (α), Cytokeratin 8, Cytokeratin 10, Loricrin | Clf‐Sdr | 252 (286) | 99 | 20 | 4 | 0.451 | 0.070 | 0.337 | −0.409 | 0.105 | 196.05 | 0.04653 |
| SesJ (S. epi) | Plasminogen | Clf‐Sdr | 213 (217) | 36 | 0 | 4 | 0.378 | 0 | 0.288 | −0.378 | 0.052 | 121.12 | 0.02044 |
| Pro‐rich | |||||||||||||
| Aap (S. epi) | Self | G5‐E | 126 (138) | 0 | 40 | 2 | 0.261 | 0.290 | 0.537 | −0.043 | 0.058 | 0.40 | 0.03154 |
| SasG | Self | G5‐E | 40 (69) | 0 | 12 | 3 | 0.362 | 0.174 | 0.476 | 0.101 | 0.096 | 1.11 | 0.07106 |
| CNA | Collagen, C1q | Clf‐Sdr | 55 (58) | 0 | 15 | 3 | 0.431 | 0.259 | 0.554 | 0.052 | 0.071 | −0.08 | 0.09578 |
| FnbpA | Fibronectin, Fibrinogen (γ) | Clf‐Sdr | 76 (108) | 0 | 36 | 2 | 0.269 | 0.333 | 0.596 | −0.046 | 0.209 | −1.76 | 0.04935 |
| FnbpB | Fibronectin, Fibrinogen (α), Elastin | Clf‐Sdr | 62 (94) | 0 | 29 | 2 | 0.277 | 0.309 | 0.584 | −0.021 | 0.205 | −1.74 | 0.05606 |
| Other | |||||||||||||
| SraP (SasA) | Sialoprotein, gp340 | SRRP | 73 (80) | 2 | 2 | 1 | 0.138 | 0.025 | 0.318 | −0.037 | 0.153 | −0.06 | 0.45721 |
| FmtB (SasB) | Unknown | Other | 57 (58) | 0 | 1 | 1 | 0.190 | 0.017 | 0.368 | 0.052 | 0.342 | −0.40 | 0.28539 |
| SasC | Self | Other | 177 (220) | 2 | 9 | 2 | 0.323 | 0.041 | 0.390 | −0.023 | 0.147 | −0.06 | 0.12394 |
a
Ligand specificities for the LPXTG proteins are according to Arrecubieta et al. (2007), Davis et al. (2001), Formosa‐Dague et al. (2016), Foster (2020), Schneewind (2019), and Yang et al. (2014a).
b
The families of CWA proteins are listed according to Foster et al. (2014), Speziale et al. (2014), and Yang et al. (2014b).
c
Sequence parameters were calculated using the CIDER server (http://pappulab.wustl.edu/CIDER/) (Das & Pappu, 2013; Martin et al., 2016).
d
f PPII was calculated using the Tomasso et al. algorithm (Tomasso et al., 2016).
e
SCD was calculated using the equation for SCD provided in Sawle and Ghosh (Sawle & Ghosh, 2015).