FIG. 1.
Hydrophobicity of native Tromp1 is conformation dependent. A total of 108 T. pallidum organisms were extracted in 0.1% TX-114 in the presence or absence of urea prior to phase separation and SDS-PAGE. Transferred immunoblots contain whole organisms (W), protoplasmic cylinders (P), aqueous-phase proteins (A), and detergent-phase (D) proteins. Panels: A, urea-untreated fractions probed with anti-Tromp1 serum (αTromp1); B, urea-treated fractions probed with αTromp1; C, the same blot as in panel B reprobed with a monoclonal antibody against the 47-kDa lipoprotein (α47-kDa LP). Numbers on the left indicate the positions of the molecular weight standards in thousands.