FIG. 6.

Molecular cross-linking of nonrenatured and renatured rTromp1. Samples containing 1 μg of nonrenatured and renatured Tromp1 were incubated in the cross-linker BS³ at the concentrations indicated. Samples were separated by SDS-PAGE, immunoblotted, and probed with anti-Tromp1 serum. Arrows indicate the molecular mass positions of monomeric rTromp1 and the positions of putative dimeric and trimeric forms of rTromp1. Numbers on the left indicate the positions of the molecular weight standards (in thousands).