FIG. 8.

Porin activity of renatured rTromp1. Renatured rTromp1 was added to the aqueous phase (1 M KCl) bathing a lipid bilayer membrane. (A) Step increases in conductance after the addition of renatured rTromp1. (B) Histogram of single-channel conductance increases for more than 104 membrane insertion events. Conductance increases showed a mean distribution about 0.65 ± 0.24 nS (± the standard deviation). No conductance activity was observed for nonrenatured rTromp1 added to the lipid bilayer membrane (data not shown).