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. 1999 Dec;181(23):7168–7175. doi: 10.1128/jb.181.23.7168-7175.1999

FIG. 9.

FIG. 9

Secondary structure analysis of nonrenatured and renatured rTromp1 by CD spectroscopy. The double minimum absorbances displayed by nonrenatured rTromp1 (solid line) is typical of proteins with a high percentage of alpha-helix. By comparison, renatured rTromp1 (dotted line) showed a distinct loss in absorbance at 222 nm, indicating a loss in alpha-helical secondary structure.