FIG. 5.

GUS activity in P. syringae pv. syringae FF5 derivatives grown in MGY broth containing sodium chloride (A), sorbitol (B), or copper sulfate (C). Prior to the GUS assays, all strains were grown for 20 h in MGY broth containing chloramphenicol. The bacterial concentration was adjusted to an A₆₀₀ of 0.1, and the cells were incubated at 250 rpm for 10 h at 28°C in MGY broth amended with NaCl, sorbitol, or copper sulfate. palgTA contains the algT promoter in the transcriptionally active orientation, and pBBR.Gus contains a promoterless glucuronidase gene. Values are the mean from one experiment containing three replicates, and the experiment was repeated with similar results. Treatments accompanied by the same lowercase letter were not significantly different at a P of 0.01 as shown by Duncan’s multiple-range test.