TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | 50 | |
| Pseudomonas syringae pv. syringae | ||
| FF5 | No detectable plasmids, nonmucoid | 29 |
| FF5.36 | Cur Kmr; contains pPSR12, slightly mucoid, nadB::Tn5 | This study |
| FF5.LK1 | Cmr; contains pPSR12, nonmucoid, algT::Cmr | This study |
| Plasmids | ||
| pBluescript SK(+) | Apr; ColEI origin, cloning vehicle | Stratagene |
| pCR2.1 | Apr Kmr; 3.9-kb cloning vector | Invitrogen |
| pRK415 | Tcr; RK2-derived cloning vector | 27 |
| pRK2013 | Kmr; helper plasmid | 17 |
| pRK7813 | Tcr; cosmid vector | 23 |
| pBBR1MCS | Cmr; 4.7-kb broad-host-range cloning vector | 31 |
| pBBR.Gus | Cmr; 6.6-kb promoter probe vector containing uidA in pBBR1MCS | 45 |
| pPSR12 | Cur Smr; 200-kb, confers constitutive alginate production to P. syringae pv. syringae FF5 | 29 |
| pSL1 | Apr Cmr; 650-bp Cmr cassette in pBluescript SK(+) | 33 |
| pMGm | Apr Gmr; 2-kb Gmr cassette | 43 |
| pJG309 | Tcr; contains algT from P. aeruginosa | 19 |
| pFF5.36 | Tcr Kmr; contains Tn5-inactivated alginate genes from FF5.36 in pRK7813 | This study |
| pFF5.36B | Apr Kmr; contains a portion of Tn5, nadB, and algT as a 6-kb BamHI fragment from pFF5.36 in pBluescript SK(+) | This study |
| pLKT5 | Tcr; cosmid clone from FF5(pPSR12) in pRK7813 | This study |
| pBTB6.5 | Apr; contains a 6.5-kb BamHI fragment from pLKT5 in pBluescript SK(+) | This study |
| pBTB.Cm | Apr Cmr; contains algT::Cmr in pBluescript SK(+) | This study |
| pRTB6.5 | Tcr; contains a 6.5-kb BamHI fragment from pLK5T5 in pRK415 | This study |
| pRTB6.5.Cm | Tcr Cmr; contains algT::Cmr in pRK415 | This study |
| pCRalgTA | AprKmr; 1.2-kb PCR fragment in pCR2.1 | This study |
| pCRalgTI | AprKmr; 1.2-kb PCR fragment in pCR2.1 | This study |
| palgTA | Cmr; 1-kb HindIII/PstI fragment containing the algT promoter region in pBBR.Gus; transcriptionally active | This study |
| palgTA.1 | Cmr Gmr; contains Gmr cassette from pMGm in palgTA | This study |
| palgTI | Cmr; 1-kb KpnI/HindIII fragment containing the algT promoter region in pBBR.Gus; transcriptionally inactive | This study |