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. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: Nat Biomed Eng. 2022 Aug 11;6(8):944–956. doi: 10.1038/s41551-022-00917-y

Fig. 4 |. DISCoVER for extraction-free detection of saliva.

Fig. 4 |

a, Direct saliva lysis conditions were tested for compatibility with the DISCoVER workflow. Replicates that did not amplify are represented at 0 min. Error bars represent mean ± s.d. of amplified technical replicates, n = 4. IA, inactivation reagent; QE, QuickExtract. b, Schematic of contrived saliva sample generation, quantification via RT–qPCR, and detection via DISCoVER to determine analytical sensitivity. c, Fold change in DISCoVER signal relative to NTC on SARS-CoV-2-positive saliva samples at 5 min of Cas13 detection. Error bars show mean ± s.d. with n = 20 biological replicates. d, Fold change in DISCoVER signal relative to NTC on 30 negative saliva samples collected before November 2019, at 5 min of Cas13 detection.