Extended Data Fig. 1 |. rLAMP with T7-Floop primer mechanism.

FIP and BIP primers, assisted by F3 and B3 primers to XX, bind to the target DNA at F2c and B2c, adding complementary regions of DNA to the amplicon (F1c and B1c). Their complementarity to F1 and B1 result in loop structures, which facilitate further FIP and BIP binding at the loops (F2c and B2c) and extension, amplifying the target via formation of long concatemers. Floop and Bloop primers can additionally bind to the loops and extend, further increasing amplification. Addition of the T7 promoter at the 5’ end of the loop primer provides a substrate for T7 polymerase to transcribe, resulting in an RNA product containing inverted repeats of the target amplicon.