TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or relevant markers | Reference or source |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-pro) [F′ proAB lacIqlacZΔM15 Tn10] | Stratagene |
| DH5α | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lacZYA-argF)U169 φ80ΔlacZΔM15 | Gibco/BRL |
| SM10 λpir | thi thr leu tonA lacY supE recA RP4-2-Tc::Mu Kmr λpir | 36 |
| P. stuartii strains | ||
| PR50 | Wild type | 44 |
| PR51 | PR50 ΔaarA | 43 |
| XD37 | PR50 cma37::mini-Tn5 lacZ1 | This study |
| XD37.A | XD37 ΔaarA | This study |
| XD67 | PR50 cma67::mini-Tn5 cat/lacZ | This study |
| XD67.A | XD67 ΔaarA | This study |
| XD156 | PR50 cma156::mini-Tn5 cat/lacZ | This study |
| XD156.A | XD156 ΔaarA | This study |
| XD227 | PR50 cma227::mini-Tn5 cat/lacZ | This study |
| XD227.A | XD227 ΔaarA | This study |
| XD230 | PR50 cma230::mini-Tn5 cat/lacZ | This study |
| XD230.A | XD230 ΔaarA | This study |
| Plasmids | ||
| pACYC184Δ1 | pACYC184 containing a ScaI-EcoRI deletion within the chloramphenicol resistance gene | This study |
| pUT::mini-Tn5 lacZ1 | Transposon delivery vector | 11 |
| pUT::mini-Tn5 cat/lacZ | Transposon delivery vector | This study |