FIG. 1.

Murein turnover in mutants with deletions in LTs. Cells were pulse labeled with [³H]GlcNAc, and the loss of radiolabel from SDS-insoluble murein during a chase was determined as described in Materials and Methods. The curves are averages of four independent experiments. The average standard deviation was less than 2%. Dotted line, MC1061 (wild type); open squares, LT12 (ΔMltA); open diamonds, MUF33 (ΔMltB); open triangles, MUF16 (ΔSlt70); solid circles, LT60 (ΔSlt70 ΔMltA); solid triangles, MUF49 (ΔSlt70 ΔMltB); open circles, MUF45 (ΔMltA ΔMltB); solid squares, MUF61 (ΔSlt70 ΔMltA ΔMltB).