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. 2023 Jul 9;14(11):2039–2050. doi: 10.7150/jca.84601

Figure 5.

Figure 5

ZFP57 transcriptionally regulated the expression of BRCA1 to regulate the cell cycle. A. Intersection of RNA-seq and CHIP-seq target genes. ChIP-Seq data using Cistrome Data Browser (CistromeDB: 73979/ 73980, 293T cells; CistromeDB: 103252/ 103252, H1, Embryonic Stem Cell.) B, Heat map of BRCA1 using our RNA-Seq data. C. BRCA1 protein expression in CAOV3 and OV429 cells expressing NC or Si-ZFP57, and OVCAR3 and OVCAR8 expressing lenti-vector or lenti-ZFP57. D. BRCA1 mRNA expression in CAOV3 and OV429 cells expressing NC or Si-ZFP57, and OVCAR3 and OVCAR8 expressing lenti-vector or lenti-ZFP57. E. The binding sites of ZFP57 to the promoter region of BRCA1 predicted by MEME and luciferase reporter assay was performed using 293T cells after transfecting the wild type plasmids and mutated plasmids (mutation site: orange). Data are presented as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001.