Fig. 4. Pharmacokinetics of BNNT/β-glucan-IgG complex in SK-OV3 xenograft model mice. (a) Biodistribution of the BNNT/β-glucan-IgG complex. The inset represents magnified graph for the accumulation in kidney, lung, liver, normal, brain, spleen and heart. Tumor-bearing mice were established through subcutaneous injection of a SK-OV-3 cell suspension at the right femur of nude mice. After 30 days incubation, the BNNT/β-glucan-IgG complex ([B] = 200 ppm, mice 100 μL) were intravenously injected. At 3, 6 and 24 h post injection, tumors, blood, and organs (kidney, lung, liver normal (skin), brain, spleen, and heart) were collected and lysed by aqua regia. The resulting solution was analyzed using ICP-AES (n = 3). Data represents mean ± SD and individual measurements. (b) The ratio of boron concentration in tumor tissue against blood. (c) The ratio of boron concentration in tumor tissue against normal tissue.