Fig. 3. (a) Grouped box plot of t1/2 values obtained for the SiNP-DNA interface assembly with a varying weight percentage (1 wt% and 10 wt%) of KRYTOX in HFE 7500 oil against concentrations of 0.5 and 10 mM NaCl. The box plot shows the median and 25–75th percentiles. Individual data points, mean and outliers are represented by hollow, red and black dots, respectively. 33 droplets were analyzed from 5 independent experiments for each condition. The corresponding mean values are also indicated above each box plot. (b) Interfacial tension (IFT) values for SiNP-DNA/KRYTOX systems obtained at the variable concentrations of KRYTOX and NaCl used in this study, as analyzed using 10–15 pendant droplets. A two-way ANOVA was performed using SPSS software to analyze the effect of CNaCl and CKRYTOX on the t1/2. The test revealed that there was no statistically significant interaction between the effects of CNaCl and CKRYTOX (p = 0.181). Simple main effects analysis showed significant effects on the t1/2 that are indicated by asterisks (***p < 0.001). Outliers were determined according to Tukey's formula [Q1 − 1.5 IQR; Q3 + 1.5 IQR].
