FIG. 4.

The effect of xonA, recJ, recG, and ruvC mutations, and the indicated double mutations, on intramolecular recombination proficiency. Substrates were released from infecting Chimera phage by in vivo restriction and samples were taken at the indicated times after infection. All wild-type derivatives were infected with λRF953, and all recD1011 derivatives were infected with λZS820 (MOI = 2). SalI-digested total cellular DNA preparations were subjected to Southern hybridization as described in Material and Methods. The kinetics of product formation were determined by phosphorimaging analysis of Southern hybridization patterns. The radioactivity is presented in arbitrary units.