TABLE 2.
Phage | oripACYC184 | Chi at A | Chi at B |
---|---|---|---|
λZS820 | 0 | 0 | 0 |
λRF949 | + | 0 | + |
λRF950 | + | + | 0 |
λRF951 | + | + | + |
λRF953 | 0 | + | + |
Each Chi site in the schematic representation of the cloned intramolecular recombination substrate consists of three Chi octamers (6). The approximate locations of oripACYC184, the Kanr gene, and Chi insertion sites (A and B) are indicated.