TABLE 3.

A Chi site on one homolog is sufficient for intramolecular recombination in recG and ruvAC mutants^a

Strain	Genotype	% Frequency (range) of Kanr recombinants
		Chi at A and B	Chi at A	Chi at B
AC227	Wild type	10.3 (10–12.1) [1.0]	3.0 (2.4–3.7) [0.3]	1.7 (1.2–2.2) [0.2]
AC297	recG162	3.5 (2.5–3.7) [1.0]	0.8 (0.6–0.9) [0.2]	0.4 (0.3–0.4) [0.1]
AC303	ΔruvAC	4.1 (3.7–6.7) [1.0]	1.4 (1.1–1.6) [0.3]	0.6 (0.5–0.9) [0.1]

^aThe approximate locations of Chi sites, ori_pACYC184 (ori), and the Kan^r gene are indicated. Kan^r recombinants were scored as described in Materials and Methods (MOI = 0.2). The median and range (in parentheses) of at least three independent experiments are presented. For each genotype, values in brackets represent the ratios of recombinant frequencies for the indicated substrate to that for the substrate with two Chi sites.