Skip to main content
. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: Anal Biochem. 2022 Aug 9;655:114844. doi: 10.1016/j.ab.2022.114844

Fig. 7.

Fig. 7.

The MS/MS spectrum for the isopeptide crosslinked peptide KGVVKAE179K/ISLVKTEFHAK113EQYR. The parent ion is at m/z 672.894, +4 charged. The upper panel shows the mass range from m/z 150 to 870. This emphasizes a 5-amino, +1, b-ion sequence (KGVVK) from the N-terminus of the green peptide; a 3-amino acid, +4, ladder sequence from the N-terminus of the green peptide; and a 2-amino acid, +3, y-ion crosslink specific sequence (VK) from the blue peptide appended by an unassigned residue V. The lower panel shows the mass range from m/z 680 to 840. This emphasizes the mixed fragmentation sequence (VKVK) from the green peptide. Mixed fragmentation peaks are marked with arrows. Unlabeled, red masses mostly represent loss of water, amine, or CO.