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. Author manuscript; available in PMC: 2023 Jul 25.
Published in final edited form as: J Biomol Struct Dyn. 2022 Mar 28;41(8):3295–3304. doi: 10.1080/07391102.2022.2050947

Figure 2: FH2 domains of formins are highly conserved.

Figure 2:

(A) Schematic representation of domains arrangement in formins: INF2, FMNL3 and Bni1P. (B) Multiple sequences alignment of the FH2 domain of the three proteins using MUSCLE [23]. Residues with 90% identity are shown in blue in (C). (C) Phylogenetic tree (Neighbor-joining) of FH2 domains and their relationship to each other. (D) The isoforms of FH2 domain are illustrated structurally in (i), (ii), and (iii); (iv) shows the superimposed FH2 domains. The structural models of Bni1p (1Y64) and FMNL3 (4EAH) were obtained from PDB. The structural model of INF2 was generated by homology modelling using PHYRE-2 [24]. To confirm the model generated by Phyre2 we used Swiss Model [33] and DTU server [34] to generate additional models (data not shown) and those were similar to the one generated by PHYRE-2.