FIG. 1.

Partial restriction map of pNLY1::PsusA, the expression vector used to express susG in trans in B. thetaiotaomicron. The selectable marker in E. coli is ampicillin resistance (bla) plus chloramphenicol resistance (CAT). The selectable marker in B. thetaiotaomicron is chloramphenicol resistance. The E. coli replication region (pBI136) and B. thetaiotaomicron replication and mobilization region (pBI143) are shown. The CAT gene is cloned downstream of the IS4351 promoter. The promoter to control expression of cloned genes was obtained by PCR of a 392-bp fragment from the B. thetaiotaomicron chromosome containing PsusA.