FIG. 2.

Immunoblot showing SusG expression from a multicopy plasmid. Approximately 50 μg of protein was loaded onto each lane. All membrane fractions were obtained from cells grown on minimal medium with maltose as the sole carbohydrate source. Lanes: 1, membrane fraction from B. thetaiotaomicron 5482; 2, membrane fraction from B. thetaiotaomicron ΩsusG(pSGC23A); 3, membrane fraction from B. thetaiotaomicron ΩsusG; 4, membrane fraction from B. thetaiotaomicron ΩsusC(pSGC23A); 5, membrane fraction from B. thetaiotaomicron ΩsusC. The arrows mark the known starch OMPs.