Table 3).

Summary of time consumed during Raman analysis scanning of four replicate filter samples in the subsampling component of the DI water study. Preparation includes placing the filter on a 5 mm × 5 mm grid sheet using Skin Tac liquid adhesive and holding it in-place on the Raman microscope stage using magnets.

Raman Time Consumption (Minutes)
		6.25%	12.5%	25%	50%	75%	100%
Sample 1	Filter Preparation	20	20	20	20	20	20
	Generating Maps	150	200	240b	170	180	150
	Scanning Timea	44	71	148	307	493	609
	Total Time	214	291	408	497	693	779
Sample 2	Filter Preparation	25	25	25	25	25	25
	Generating Maps	40	90	180	190	160	170
	Scanning Time	44	59	133	361	429	466
	Total Time	109	174	338	576	614	661
Sample 3	Filter Preparation	15	15	15	15	15	15
	Generating Maps	40	100	190	180	170	160
	Scanning Time	43	66	129	289	410	529
	Total Time	98	181	334	484	595	704
Sample 4	Filter Preparation	20	20	20	20	20	20
	Generating Maps	30	90	140	150	170	170
	Scanning Time	51	83	170	294	360	420
	Total Time	101	193	330	464	550	610
Average Time Consumption (min)		131	210	353	505	613	689
Standard Deviation		55.9	54.7	37.1	49.1	59.7	71.5
Standard Error		27.9	27.4	18.6	24.5	29.9	35.8

^aScanning time depends on the number of particles. Normally, it requires 1.2 minutes to scan one particle.

^bTechnical difficulties – the computer and instrument needed to be restarted several times.