FIG. 2.

Formation of SDS-resistant multimers and yields of representative PulD variants obtained by linker insertions in the pulD gene. Three categories of mutants were obtained: slightly reduced total yields and unaltered multimerization (B), slightly reduced yields and substantially reduced multimerization (C), and slightly reduced yields and abolished or drastically reduced multimerization (D) compared with normal PulD (A). Samples from IPTG-induced and noninduced cells of E. coli expressing pulS together with the pulD variants under lacZp control on pSU18 derivatives were examined by SDS-PAGE and immunoblotting. The same amount of total cell extract was loaded in each lane, and each panel represents the same chemiluminescence exposure time.