TABLE 1.
Plasmids used in this study
| Plasmida | Antibiotic resistance | Vector | Cloned gene(s) and mutations | lacZp control | Reference |
|---|---|---|---|---|---|
| pCHAP231 | Ap | pBR322 | All pul genes | − | 9 |
| pCHAP1226 | Ap | pBR322 | pCHAP231 ΔpulD pulB::kan1 | − | 27 |
| pCHAP1106 | Km | pBGS18 | malE′-′pulA | + | 25 |
| pCHAP3072 | Km | pBGS18 | pulAAB-′celZ | + | This study |
| pCHAP3671 | Ap | pUC18 | pulD | + | This study |
| pCHAP3635 | Cm | pSU18 | pulD | + | This study |
| pCHAP1349 | Cm | pSU18 | phoE′-′pulD | + | This study |
| pCHAP3608 | Cm | pSU18 | outD | + | This study |
| pCHAP1300 | Cm | pSU18 | pulA′-′pulD | + | This study |
| pCHAP585 | Ap | pUC19 | pulS | + | This study |
a
pCHAP3635 and pCHAP3671 carry the same pulD fragment as pCHAP362 (8). The soluble PulA encoded by pCHAP1106 lacks the fatty acylated N terminus normally found on this protein. pCHAP3072 is the same as pCHAP3044 (33) except that gene celZ encoding the E. chrysanthemi exoprotein CelZ (32) replaces blaM. The hybrid protein encoded by this gene is fully and efficiently secreted with the pullulanase secreton. pCHAP585 carries the same pulS gene fragment as pCHAP378 (7).