TABLE 2.

Effects of 24-amino-acid insertions at different sites in PulD

Insertion no.	Position (aa)a	Domainb	No. of examples tested	Yield and multimerizationc	% Complementationd
0				A	100
1	22		1	C	0
2	58	N	1	C	0
3	77	N	1	C	0
4	117	N	1	C	0
5	122	N	2	C	0
6	135	N	5	C	0
7	156	N	2	C	0
8	196	N	1	C	0
9	267	N	3	C	0
10	281	N	1	D	0
11	290	N	1	D	0
12	318	β	3	D	0
13	406	β	2	C	0
14	410	β	7	C	0
15	441	β	2	D	0
16	465	β	2	D	0
17	507	β	1	D	0
18	533	β	1	D	0
19	640	S	1	B	22

^aPositions are the last amino acid in the sequence of prePulD before the insertion site. 

^bN, N domain; S, S domain. 

^cYields and multimerization are classified into four categories, as illustrated in Fig. 2. 

^dComplementation tests were performed in maltose-induced cultures of PAP7447 (ΔpulD in chromosome). The results are averages of at least three independent assays.