TABLE 3.

Characterization of PulD-OutD and OutD-PulD secretin chimeras

Proteina	Plasmid	Yieldb	Predominant form at 30°Cc	% Complementationd
PulD (P)	pCHAP3635	Normal	Multimer	100
OutD (O)	pCHAP3608	Reduced	Monomer	0
P273O	pCHAP3637	Normal	Monomer	0
O273P	pCHAP3662	Reduced	Monomer	55
O418P	pCHAP3615	Reduced	Monomer	0
P365O	pCHAP3643	Normal	Monomer	0
P67O	pCHAP3638	Reduced	Monomer	0
P158O	pCHAP3652	Normal	Monomer	0
O158P	pCHAP3650	Normal	Multimer	100
P67O158P	pCHAP3648	Normal	Multimer	100
O158P273O	pCHAP3658	Reduced	Monomer	0
P158O273P	pCHAP3665	Normal	Multimer	100

^aJunctions between the two proteins in the chimeras are indicate as amino acid residues in the sequence of the protein segment upstream. P, PulD; O, OutD. 

^bYields were estimated from immunoblots with PulD antibodies. Normal, close to yield of PulD under identical conditions; reduced, <20% of normal yield. 

^cThe multimer-monomer ratio was determined in samples that were not heated to 100°C before electrophoresis. The form of the protein that predominates under these conditions is indicated. Where monomer is indicated, the multimer was barely or totally undetectable by immunoblotting. 

^dComplementation tests were performed in maltose-induced cultures of PAP7447 (ΔpulD in chromosome). The results are averages of at least three independent assays.