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. 2023 Jul 25;18(7):e0289003. doi: 10.1371/journal.pone.0289003

Table 2. Percent mortality of neonates (on leaf disk) and L2 larvae (on square) of H. armigera, H. zea and the F1 progeny from ♀H. armigera × ♂H. zea after 5 d on leaves of Bt and non-Bt cotton technologies in laboratory trials.

Pest species and cotton technologies n a Leaf disk (growth stage 15)b Leaf disk (growth stage 55)b Square (growth stage 55)b
Dead % mortality (95% CI) Dead % mortality (95% CI) Dead % mortality (95% CI)
Helicoverpa armigera (Ha-Lab)
DAS-21023-5 × DAS-24236-5 × SYN-IR102-7 128 110 85.9 (79.0–91.1) a 92 71.9 (63.6–79.1) a 70 54.7 (46.0–63.1) a
DAS-21023-5 × DAS-24236-5 128 93 72.7 (64.4–79.8) a 86 67.2 (58.7–74.9) a 77 60.2 (51.5–68.3) a
Non-Bt cotton 128 3 2.3 (0.7–6.5) b 3 2.3 (0.7–6.5) b 1 0.8 (0.1–4.4) b
Helicoverpa zea (Hz-Lab)
DAS-21023-5 × DAS-24236-5 × SYN-IR102-7 128 128 100.0 (97.2–100.0) a 128 100.0 (97.2–100.0) a 128 100.0 (97.2–100.0) a
DAS-21023-5 × DAS-24236-5 128 128 100.0 (97.2–100.0) a 53 41.4 (33.1–50.0) b 70 54.7 (46.0–63.1) b
Non-Bt cotton 128 16 12.5 (7.6–19.2) b 10 7.8 (4.1–13.6) c 14 10.9 (6.4–17.3) c
F1 hybryd (♀ Ha-Lab × ♂ Hz-Lab)c
DAS-21023-5 × DAS-24236-5 × SYN-IR102-7 128 128 100.0 (97.2–100.0) a 97 75.8 (67.8–82.3) a 48 37.5 (29.5–46.1) a
DAS-21023-5 × DAS-24236-5 128 59 46.1 (37.6–54.8) b 51 39.8 (31.7–48.5) b 15 11.7 (7.0–18.2) b
Non-Bt cotton 128 14 10.9 (6.4–17.3) c 16 12.5 (7.6–19.2) c 3 2.3 (0.7–6.5) c

aNumber of larvae tested per growth stage.

bGrowth stage number according to Munger et al. [28]. Means (95% CI) followed by the same letter in each column and species are not significantly different due to nonoverlap of 95% CIs.

cOnly the crossing of ♀H. armigera × ♂H. zea produced fertile eggs allowing bioassays to be carried out.