Fig 2. Inactivation of pgl impacts pyrimidine biosynthesis.
RNA-seq analysis of the mutation in pgl compared to WT S. aureus. A) Volcano plot of differentially expressed genes identified from RNA-seq comparing WT and pgl transcriptomes. B) qRT-PCR was conducted on each differentially expressed gene identified from RNA-seq analysis. Correlation plot between independent RNA-seq and qRT-PCR data. n = 6.