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(A) Calcium transients (ΔF/Fθ) measured using GCaMP5G in response to drug application. Vehicle (control), choline (10 mM), and choline with α-bungarotoxin (100 nM, BTX). (B) Average calcium peaks across experimental groups. Choline with pertussis toxin (5 μg/ml, PTX) (n = 21–25 cells). Statistical significance: *p<0.05; **p<0.01, ***p<0.001.
