Fig 6. G-protein inhibitors block choline-mediated MCF-7 cell proliferation.

Approximately 10⁴ MCF-7 cells were seeded into microwell plates for 3 days then assessed via an MTT assay: (A) vehicle (control), pertussis toxin (5 μg/ml, PTX), choline (10 mM), and choline with PTX, (B) vehicle (control), YM 254890 (1 μM), choline (10 mM), and choline with YM 254890. Bars represent means ± SEM of 3 independent experiments. Asterisk denotes significant difference from choline treated group (p < 0.05).