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. 2023 Jul 25;12:e83884. doi: 10.7554/eLife.83884

Figure 4. Lysate supplementation rescues luminal ER protein dynamics but not ER membrane protein dynamics.

Figure 4.

(A) FRAP curve of intestinal ER-transmembrane protein, SPCS-1::GFP, from day 1 adult animals grown on Empty Vector (EV) or let-767 RNAi mixed with EV in a 1:1 ratio supplemented with vehicle or N2 lysate (geometric mean for n=20 pooled from two biological replicates). Lines represent geometric standard deviation. (B) Calculated percent mobile SPCS-1::GFP of (A). Lines represent geometric mean and geometric standard deviation. Mann-Whitney test p-value ****<0.0001. (C) FRAP curve of intestinal ER-lumen protein, mRuby::HDEL, from day 1 adult animals grown on EV or let-767 RNAi mixed with EV in a 1:1 ration supplemented with vehicle or N2 lysate (geometric mean for n=20 pooled from two biological replicates). Lines represent geometric standard deviation. (D) Calculated percent mobile mRuby::HDEL of (A). Lines represent geometric mean and geometric standard deviation. Mann-Whitney test p-value ****<0.0001 and *<0.05.