Video 1. MS2 live imaging of hbA enhancer reporter.

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Live imaging of a ‘hbA >MS2-yellow; aTub >MCP-mEmerlad’ Tribolium embryo during the blastoderm stage. Nuclear localization signal (NLS) signal within the aTub >MCP-mEmerald construct mediates a weak and diffuse mEmerald signal within nuclei. Upon transcription, MS2 loops within the hbA >MS2-yellow construct recruit MCP-mEmerald fusion proteins at transcription sites, resulting in mEmerald bright puncta. Here bright mEmerald puncta are observed throughout the posterior end of the blastoderm, reflecting transcriptional activity of enhancer hbA in the early Tribolium embryo. Posterior to the right.