Video 4. MS2 live imaging of runB enhancer reporter.

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Live imaging of a ‘runB >MS2-yellow; aTub >MCP-mEmerlad’ Tribolium embryo during the blastoderm stage. Nuclear localization signal (NLS) signal within the aTub >MCP-mEmerald construct mediates a weak and diffuse mEmerald signal within nuclei. Upon transcription, MS2 loops within the runB >MS2-yellow construct recruit MCP-mEmerald fusion proteins at transcription sites, resulting in mEmerald bright puncta. Here bright mEmerald puncta are observed initially to be distributed as a posterior cap that eventually propagates towards the anterior to form a stable band. Posterior to the right.