| Recombinant DNA reagent |
pETDuet-1Δ6His_hNMT_Nef |
DOI:10.1371/journal.pone.0010081
|
|
Provided by Prof. Willbold (Forschungszentrum Jülich, Germany) |
| Peptide, recombinant protein |
Bovine serum albumin (BSA) |
Sigma-Aldrich |
Cat.# A7906 |
For production of mCHP3 and mRec |
| Peptide, recombinant protein |
Trypsin |
Sigma-Aldrich |
Cat.# T1426 |
For limited trypsinolysis |
| Chemical compound, drug |
Myristic acid |
Carl Roth GmbH |
Cat.# 6469.1 |
For production of mCHP3 and mRec |
| Chemical compound, drug |
POPC |
Avanti Polar Lipids, Inc |
Cat.# 850457C |
Liposome preparation |
| Chemical compound, drug |
POPS |
Avanti Polar Lipids, Inc |
Cat.# 840034C |
Liposome preparation |
| Chemical compound, drug |
ProteOrange |
Lumiprobe |
Cat.# 40210 |
Dye for FPH assay |
| Commercial assay, kit |
Monolith Series Protein labeling kit – RED-NHS |
Nanotemper Technologies |
Cat.# MO-001 |
MST labeling kit |
| Commercial assay, kit |
Monolith Series Premium Capillaries |
Nanotemper Technologies |
Cat.# MO-K025 |
MST capillaries |
| Software, algorithm |
Data2Dynamics Software |
https://github.com/Data2Dynamics/d2d; Kreutz, 2023
|
|
For statistical analysis of datasets consisted of technical and biological replicates |
| Other |
16% Novex Tricine Gel |
Thermo Fisher Scientific |
Cat.# EC66955BOX |
SDS–PAGE system for separation of CHP3 and mCHP3 |
| Other |
96-well black plates |
Greiner Bio-One |
Cat.# 655076 |
For FPH assay |
