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Manual versus MFED enrichment of E1 over Pep2. Selection was performed on various starting ratios of E1:Pep2 (1:2, 1:38, 1:67, 1:135, and 1:292). Resulting PCR products were run on agarose gels (A and S3). Gel images were analyzed to extract molar ratios before and after selection (B and S3). MFED selection consistently outperformed manual selection, averaging 13-fold enrichment compared to 5-fold enrichment observed with manual selection (* indicates p<0.05).
