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Plasmid pSEC4 was constructed by inserting into the autonomously replicating C. albicans plasmid pVEC (i) 1.0 kb of genomic DNA immediately 5′ to the C. albicans GAL1 start codon and (ii) the ORF and transcription terminator from C. albicans SEC4. pS28N was identical to pSEC4 except that the sec4(S28N) allele replaced wild-type SEC4.
