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The deduced Sec4p’s of C. albicans (Ca) and S. cerevisiae (Sc) were 61% identical (:). Conserved GTP-binding (∗) and membrane attachment (+) domains are shown, as are the positions where a G147→D substitution causes a temperature-sensitive mutation (▵) and where S34→N and N133→I substitutions cause dominant-negative mutations (↑) in S. cerevisiae Sec4p.
