Table of Materials
Name | Company | Catalog Number | Comments |
---|---|---|---|
2.5 lb weight | CAP Barbell | RP–002.5 | |
Acrylic sheets (6 in × 4 in × 3/8 in) | Falken Design | ACRYLIC–CL–3–8/1224 | Large sheet cut to smaller sizes |
Ampicillin sodium salt | Sigma-Aldrich | A9518 | |
Autoclavable squeeze bottle | Nalgene | 2405–0500 | |
Bacto agar | BD Difco | 214030 | |
Bacto peptone | Thermo Scientific | 211677 | |
Basin, 25 mL | VWR | 89094–664 | Disposable pipette basin |
Cabinet style vacuum desiccator | SP Bel-Art | F42400–4001 | Do not need to use dessicant, only using as a vacuum chamber. |
CaCl2 | Acros Organics | 349615000 | |
Caenorhabditis elegans N2 | Caenorhabditis Genetics Center (CGC) | N2 | Wildtype strain |
Carbenicillin | GoldBio | C–103–25 | |
Centrifuge | Beckman | 360902 | |
Cholesterol | ICN Biomedicals Inc | 101380 | |
Compressed oxygen tank | Airgas | UN1072 | |
CuSO4 | Fisher Chemical | C493–500 | |
Dry bead bath incubator | Fisher Scientific | 11–718–2 | |
Escherichia coli OP50 | Caenorhabditis Genetics Center (CGC) | OP50 | Standard labratory food for C. elegans |
Ethanol | Millipore | ex0276–4 | |
Floxuridine | Research Products International | F10705–1.0 | |
Hybridization oven | Techne | 731–0177 | Used to cure PDMS mixture, any similar oven will suffice |
Incubators | Shel Lab | 2020 | 20 °C incubator for maintaining worm strains and 37 °C incubator to grow bacteria |
Isopropyl ß-D-1-thiogalactopyranoside (IPTG) | GoldBio | I2481C100 | |
K2HPO4 | Fisher Chemical | P288–500 | |
KH2PO4 | Fisher Chemical | P286–1 | |
Kimwipes | KimTech | 34155 | Task wipes |
LB Broth, Lennox | BD Difco | 240230 | |
Low melt agarose | Research Products International | A20070–250.0 | |
MgSO4 | Fisher Chemical | M–8900 | |
Microwave | Sharp | R–530DK | |
Multichannel repeat pipette, 20–200 μL LTS EDP3 | Rainin | 17013800 | The exact model used is no longer sold, a similar model's catalog number has been provided |
NaCl | Fisher Bioreagents | BP358–1 | |
Nunc OmniTray | Thermo Scientific | 264728 | Clear polystyrene trays |
Parafilm M | Fisher Scientific | 13–374–10 | Double-wide (4 in) |
Petri plate, 100 mM | VWR | 25384–342 | |
Petri plate, 60 mM | Fisher Scientific | FB0875713A | |
Plasma cleaner | Plasma Etch, Inc. | PE–50 | |
PLATINUM vacuum pump | JB Industries | DV–142N | |
PolyJet 3D printer | Stratasys | Objet500 Connex3 | PolyJet 3D printing services provided by ProtoCAM (Matrial: Vero Rigid; Finish: Matte; Color: Gloss; Resolution: X-axis: 600 dpi, Y-axis: 600 dpi, Z-axis: 1600 dpi) |
Shaking incubator | Lab-Line | 3526CC | |
smartSpatula | LevGo, Inc. | 17211 | Disposable spatula |
Superabsorbent polymer (AgSAP Type S) | M2 Polymer Technologies | Type S | Referred to in main text as "water crystals" |
SYLGARD 184 Silicone Elastomer base | The Dow Chemical Company | 2065622 | |
SYLGARD 184 Silicone Elastomer curing agent | The Dow Chemical Company | 2085925 | |
Syringe filter (0.22 μm) | Nest Scientific USA Inc. | 380111 | |
Syringe, 10 mL | Fisher Scientific | 14955453 | |
TWEEN 20 | Thermo Scientific | J20605–AP | Detergent |
Vacuum pump oil | VWR | 54996–082 | |
VeroBlackPlus | Stratasys | RGD875 | Rigid 3D printing filament |
Weigh boat | Thermo Scientific | WB30304 | Large enough for PDMS mixture volume |