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. Author manuscript; available in PMC: 2023 Dec 11.
Published in final edited form as: J Vis Exp. 2022 Dec 9;(190):10.3791/64681. doi: 10.3791/64681

Table of Materials

Name Company Catalog Number Comments
2.5 lb weight CAP Barbell RP–002.5
Acrylic sheets (6 in × 4 in × 3/8 in) Falken Design ACRYLIC–CL–3–8/1224 Large sheet cut to smaller sizes
Ampicillin sodium salt Sigma-Aldrich A9518
Autoclavable squeeze bottle Nalgene 2405–0500
Bacto agar BD Difco 214030
Bacto peptone Thermo Scientific 211677
Basin, 25 mL VWR 89094–664 Disposable pipette basin
Cabinet style vacuum desiccator SP Bel-Art F42400–4001 Do not need to use dessicant, only using as a vacuum chamber.
CaCl2 Acros Organics 349615000
Caenorhabditis elegans N2 Caenorhabditis Genetics Center (CGC) N2 Wildtype strain
Carbenicillin GoldBio C–103–25
Centrifuge Beckman 360902
Cholesterol ICN Biomedicals Inc 101380
Compressed oxygen tank Airgas UN1072
CuSO4 Fisher Chemical C493–500
Dry bead bath incubator Fisher Scientific 11–718–2
Escherichia coli OP50 Caenorhabditis Genetics Center (CGC) OP50 Standard labratory food for C. elegans
Ethanol Millipore ex0276–4
Floxuridine Research Products International F10705–1.0
Hybridization oven Techne 731–0177 Used to cure PDMS mixture, any similar oven will suffice
Incubators Shel Lab 2020 20 °C incubator for maintaining worm strains and 37 °C incubator to grow bacteria
Isopropyl ß-D-1-thiogalactopyranoside (IPTG) GoldBio I2481C100
K2HPO4 Fisher Chemical P288–500
KH2PO4 Fisher Chemical P286–1
Kimwipes KimTech 34155 Task wipes
LB Broth, Lennox BD Difco 240230
Low melt agarose Research Products International A20070–250.0
MgSO4 Fisher Chemical M–8900
Microwave Sharp R–530DK
Multichannel repeat pipette, 20–200 μL LTS EDP3 Rainin 17013800 The exact model used is no longer sold, a similar model's catalog number has been provided
NaCl Fisher Bioreagents BP358–1
Nunc OmniTray Thermo Scientific 264728 Clear polystyrene trays
Parafilm M Fisher Scientific 13–374–10 Double-wide (4 in)
Petri plate, 100 mM VWR 25384–342
Petri plate, 60 mM Fisher Scientific FB0875713A
Plasma cleaner Plasma Etch, Inc. PE–50
PLATINUM vacuum pump JB Industries DV–142N
PolyJet 3D printer Stratasys Objet500 Connex3 PolyJet 3D printing services provided by ProtoCAM (Matrial: Vero Rigid; Finish: Matte; Color: Gloss; Resolution: X-axis: 600 dpi, Y-axis: 600 dpi, Z-axis: 1600 dpi)
Shaking incubator Lab-Line 3526CC
smartSpatula LevGo, Inc. 17211 Disposable spatula
Superabsorbent polymer (AgSAP Type S) M2 Polymer Technologies Type S Referred to in main text as "water crystals"
SYLGARD 184 Silicone Elastomer base The Dow Chemical Company 2065622
SYLGARD 184 Silicone Elastomer curing agent The Dow Chemical Company 2085925
Syringe filter (0.22 μm) Nest Scientific USA Inc. 380111
Syringe, 10 mL Fisher Scientific 14955453
TWEEN 20 Thermo Scientific J20605–AP Detergent
Vacuum pump oil VWR 54996–082
VeroBlackPlus Stratasys RGD875 Rigid 3D printing filament
Weigh boat Thermo Scientific WB30304 Large enough for PDMS mixture volume