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. 2023 Jul 25;82(4):317–332. doi: 10.1016/j.jacc.2023.05.031

Figure 4.

Figure 4

miR-92a-3p Downregulates Protein Levels of Wnt5a in Cardiomyocytes

(A) Prediction of miR-92a-3p binding to phosphatase and tensin homolog (PTEN), PH domain and leucine-rich repeat protein phosphatase 2 (PHLPP2), and wingless-type MMTV integration site family, member 5a (Wnt5a) 3′-untranslated regions (UTRs) assessed by using TargetScan software version 7.2 (TargetScan). Transfection of H9c2 cardiomyocytes with miR-92a-3p mimic resulted in the reduction of protein levels of PHLPP2 (C) and Wnt5a (E), but not PTEN (B), compared with the miR-NC (n = 4-6). (D and F) Patients with high levels (above the median) of miR-92a-3p in EAT had lower myocardial expression of WNT5A (but not PPLPP2) (n = 71). Data are presented as median (25th-75th percentile). Lines on scatterplots represent medians. ∗P < 0.05 vs control by Wilcoxon signed-rank test. ∗∗P < 0.01 by Mann-Whitney U test. GAPDH = glyceraldehyde 3-phosphate dehydrogenase; other abbreviations as in Figures 1 and 3.