Figure 6.

Wnt5a Increases O₂^.– Generation in Cardiomyocytes Through Rac1-Mediated NOX Activity

(A and B) Overexpression of FLAG-tagged Wnt5a in H9c2 cells was evaluated by immunofluorescence with anti-FLAG antibody (red) and compared with H9c2 cells transfected with an empty vector (EV); nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (C to E) Basal, NADPH-stimulated, and VAS2870-inhibtable O₂^.– in H9c2 cells overexpressing or not FLAG-tagged Wnt5a (n = 8). (F to H) O₂^.– production in H9c2 cardiomyocytes in the presence/absence of WNT5A and SFRP5 (n = 5). (I to K) O₂^.– production in H9c2 cardiomyocytes treated with or without WNT5A and NSC23766, a specific RAC1 inhibitor (n = 5-7). Lines represent medians. ∗P < 0.05, ∗∗P < 0.01 vs control by Wilcoxon signed-rank test. Abbreviations as in Figures 1 and 4.