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. 2023 Jun 22;4(7):1036–1052. doi: 10.1038/s43018-023-00576-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 9 — CyCIF imaging of regions of specimen C06 which had a high fraction of Topic 7 cells. The CyCIF image is from a non-adjacent section to that used for Orion data section. Images show DNA (Sytox), ZEB1, α-SMA, NA-K ATPase, pan-cytokeratin. Location of insets are indicated. Scalebars, 2 mm, 0.5 mm, 50 µm, as indicated. The mesenchymal differentiation/EMT-marker ZEB1 (nuclear blue signal) is present in stromal cells (white arrow) but absent in the tumor cells (marked by pan-cytokeratin, red; yellow arrow). Images here are from one sample (C06).