Extended Data Fig. 2. Gating strategies for flow cytometry identification of pro-B-cell tumors.

(a) Gating strategy for identifying pro-B-cell tumors expressing the FRET-based caspase 3 reporter (named DEVD) and tumor apoptosis in the bone marrow of tumor-bearing mice. CFP and YFP are linked by the target peptide DEVD, which is cleaved upon caspase 3 activation, resulting in loss of FRET in apoptotic cells. (b) Gating strategy for identifying CD45.1⁺ IFN-γ-R1^+/+ and CD45.2⁺ IFN-γ-R1^−/− pro-B-cell tumors in the bone marrow of tumor-bearing mice. (c) Gating strategy for identifying CFP⁺, GFP⁺, YFP⁺ and mTom⁺ pro-B-cell tumors in the bone marrow of tumor-bearing mice.