Extended Data Fig. 8. IFNRs loss protects RCC from deleterious effects of IFN type I and II treatment on cell proliferation and survival.
a-d) Growth curves of 16q loss and 16q euploid RCC lines with or without knockout of Ifnar1 or Ifngr2 treated with mouse IFN-gamma, IFN-alpha or untreated. A beneficial effect of interferon receptor loss can be appreciated when 16q euploid cells are treated with IFN-alpha or IFN-gamma, data are presented as mean values +/- SD (N = 8 measurements of replicates per each time point), p values = 0.012, 0.0002 and 4.29*10-9 (c), p values 0.035 and 0.007 (d). e-h) Representative colony pictures (left) and relative quantification bar graphs (right) of 16q loss and 16q euploid RCC lines with or without knockout of Ifnar1 or Ifngr2 treated with mouse IFN-gamma, IFN-alpha or untreated. Loss of either Ifngr2 or Ifnar1 resulted in a beneficial long-term proliferative advantage only in 16q euploid RCC lines, data are presented as mean values +/- SD (N = 6 measurements of replicates per each condition), p values = 0.026 and 4.80*10-9 (g), p values = 0.018 and 3.28*10-8 (h). i-l) Western blots showing evidence of reduced STAT1 phosphorilation under interferon administration in cell lines knocked-out for either Ifngr2 or Ifnar1. Images representative of N = 3 independent experiments. * P < 0.05; ** P < 0.01; ***P < 0.001; **** P < 0.0001 by two-way ANOVA with multiple t-test (a-h).