Table 2.

List of markers used for immunohistochemistry 

Marker	Concentration/dilution	Vendor	Cat. number	Description	Target
Ki67	1:200	Abcam, Berlin, Germany	Ab15580	Polyclonal antibody	Proliferating cells
Laminin	1:100	Invitrogen, Carlsbad, CA, USA	PA184171	Polyclonal antibody	Bruch’s membrane
Collagen IV	1:100	Abcam, Berlin, Germany	Ab1217147	Polyclonal antibody	Bruch’s membrane, vasculature
Pan cytokeratin	1:100	Bio-Techne, Wiesbaden, Germany	NBP2-44,368–0.1 mg	Monoclonal antibody, Clone PAN-CK	Epithelial cells, RPE
ZO-1	1:100	Invitrogen, Carlsbad, CA, USA	339,100	Monoclonal antibody, Clone 1A12	Tight junctions
Isolectin B4 DyLight594	1:100	Vector Laboratories Inc., Burlingame, CA, USA	VEC-DL-1207	Lectin from the plant Griffonia simplicifolia. Binds to α-galactose residues on proteins and lipids	Endothelial cells, macrophages, and microglia
4′, 6-diamidino-2-phenylindole (DAPI)	1 µg/ml	Invitrogen, Carlsbad, CA, USA	10,184,322	Binds to AT rich regions of DNA	Cell nuclei