Fig. 1.

The association of Jacob with the nuclear envelope is dynamic. a The schematic represents the timeline of the FRAP experiment. b Representative confocal image sequence showing hippocampal neuron (DIV17) expressing Jacob-GFP enriched at the NL. Two pre-bleach images, the immediate post-bleach image (1 s) and multiple images taken during the recovery period, are shown. Dashed lines denote the photobleached regions of interest (ROIs). Scale bar = 10 μm. c Fluorescence recovery kinetics of Jacob-GFP at the NL in neurons (n = 15). Fluorescence intensities were background corrected, normalized, and plotted versus time (seconds). The dark violet plot represents the mean values of recovery (± SEM); light violet lines represent individual FRAP measures