FIG. 1.

Disruption of CaSLN1, CaNIK1, and CaHK1 in C. albicans. The strategy for disrupting each histidine kinase gene is illustrated with the expected structure of each product. The entire open reading frames of CaSLN1, CaNIK1, and CaHK1 were cloned into pUC19. Then, the 0.6-kb SnaBI-BalI region of CaSLN1 (16), the 1.4-kb BalI-BalI region of CaNIK1, and the 3.5-kb HpaI-BstPI region of CaHK1 were replaced by the hisG-URA3-hisG module (9). H⁵¹⁹ and D¹³⁰⁴ of CaSln1p, H⁵¹⁰ and D⁹²⁴ of CaNik1P, and H²⁰⁰⁷ and D²³⁹⁴ of CaHk1p are the predicted phosphorylating histidines and aspartic acids, respectively, of their products. TM, transmembrane domain; HK, histidine kinase domain; REC, response receiver domain; WD-REP, repeats of an approximately 90-amino-acid motif; S/T-K like, serine/threonine kinase-like domain; aa, amino acids.