Fig. 2.

Propofol inhibits the stemness of colon cancer cells and Wnt/β-catenin pathway in vitro and in vivo. A Analysis of the self-renewal abilities of LOVO and SW480 cells after treatment with or without propofol. Scale bar = 100 μm. B The expression of CD24 and CD44 in LOVO and SW480 cells was measured by flow cytometry analysis. C Western blot analysis for expression of Wnt/β-catenin signaling pathway. D Western blot analysis and qPCR after treatment with or without propofol. E The in vivo effect of propofol treatment was evaluated in xenograft mouse models. Tumour volume and weight were measured for each mouse. F IHC detection of Ki‐67, N‐cadherin and CD44 is shown. G, H-score of IHC. Scale bar = 50 μm. *P < 0.05