Fig. 4.

Propofol down-regulates SIRT1 expression, and SIRT1 is involved in stemness and EMT through Wnt/β-catenin signaling pathway and PI3K/AKT/mTOR signaling pathway. A Level of SIRT1 in LOVO and SW480 cells after propofol treatment measured by Western blot analysis and qPCR. B Analysis of the self-renewal abilities of LOVO and SW480 cells expressing mock, siSIRT1, empty and plasmid DNA SIRT1. Scale bar = 100 μm. C Expression of stem cell markers in LOVO and SW480 cells was detected by Western blot and qPCR. D and E. The invasion and migration ability of LOVO and SW480 cells were inspected by Transwell assays after downregulated and upregulated SIRT1. Scale bar = 100 μm. F Expression of EMT markers was detected by Western blot and qPCR. G, H Western blot of PI3K/AKT/mTOR signaling pathway and Wnt/β-catenin signaling pathway in LOVO and SW480 cells *P < 0.05