Fig. 6.

SIRT1 is critical in propofol-mediated stemness and EMT. A Analysis of the self-renewal abilities of LOVO and SW480 cells expressing in control, propofol, SIRT1 and SIRT1 + propofol. Scale bar = 100 μm. B Expression of stem cell markers in LOVO and SW480 cells was detected by Western blot and qPCR. C and D The invasion and migration ability of LOVO and SW480 cells were inspected by transwell assays. Scale bar = 100 μm. E Expression of EMT markers in LOVO and SW480 cells was detected by western blot and qPCR. F Expression of PI3K/AKT/mTOR signaling pathway and Wnt/β-catenin signaling pathway was detected by Western blot. G Analysis of the effect of Wnt/β-catenin pathway inhibitor Wnt-C59 on the self-renewal ability of propofol-treated cells and control cells (scale bar, 100 μm). H Analysis of the effect of PI3K/AKT/mTOR pathway inhibitor MK-2206 on invasion and migration ability of propofol-treated cells and control cells by Transwell assays. Scale bar = 100 μm *P < 0.05